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KMID : 0380220070400040571
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Journal of Biochemistry and Molecular Biology
2007 Volume.40 No. 4 p.571 ~ p.576
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Identification and Functional Analysis of LsMNPV Anti-apoptosis Genes
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Kim Yu-Sin
Xiao Hua-Zhong
Du En-Qi
Cai Guo-Shuai
Lu Song-Ya
Qi Yi-Peng
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Abstract
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Three anti-apoptosis genes, Ls-iap2, iap3 and p49 were found in Leucania separata multiple nuclear polyhedrovirus. Amino acid sequence homology of Ls-IAP2 and Ls-IAP3 with Op-IAP2 and Op-IAP3 from Orgyia pseddotsugata MNPV were 20% and 42%, while that of Ls-P49 is 28% with Sl-P49 from Spodoptera littorolis MNPV. Ls-IAP2 contains one baculoviral IAP repeat (BIR) domain followed by a RING domain, while Ls-IAP3 contains two BIRs and a RING. Ls-P49 contains a reactive site loop, predicted cleavage site (KKLD74↓G) that is different from Sl-P49 (TVID94↓G). Expressed Ls-iap3 or Ls-p49 under presence of actinomycin D in SF9 cells, DNA ladder assay revealed that Ls- IAP3 or Ls-P49 could block the apoptosis of SF9 cells induced by actinomycin D. Replication of p35 deficient-mutant Autographa californica MNPV in SF9 cells was also rescued when Ls-iap3 or Ls-p49 was expressed transiently. No anti-apoptotic activity was observed for Ls-IAP2. The results showed that both of Ls-IAP3 and Ls-P49 were functional apoptotic suppressors in SF9 cells.
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KEYWORD
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Anti-apoptosis, LsMNPV, Ls-IAP, Ls-p49
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